GuideAugust 14, 2026 · 1 min read

TB-500 Dosage and Reconstitution Math: 5 mg Vial in the Lab

Research compounds for laboratory use only · not medical advice

TB-500 vial math — reconstituting a 5 mg thymosin beta-4 fragment vial, mg to mL and U-100 unit conversions, and common research dose ranges.

TB-500 (thymosin beta-4 fragment) ships as a lyophilized research peptide in a 5 mg vial (10 vials per box) — the same 5 mg format as BPC-157, so the vial math is interchangeable.

Reconstitution table

Water added Stock concentration 500 mcg 1 mg 2.5 mg
1 mL 5 mg/mL 100 µL (20 units) 200 µL (40 units) 500 µL (100 units)
2 mL 2.5 mg/mL 200 µL (40 units) 400 µL (80 units) 1 mL (200 units)
5 mL 1 mg/mL 500 µL (100 units) 1 mL (200 units) 2.5 mL

Use bacteriostatic water (0.9% benzyl alcohol). TB-500 is soluble in water and PBS; roll the vial to dissolve, no shaking.

Dose ranges in the research literature

TB-500 protocols in the literature commonly use 500 mcg to 2.5 mg per administration in animal models, depending on the model and route. For in vitro work, dilute from the stock to the assay-defined concentration; the molecular weight calculator handles molar conversions (4963.4 g/mol).

Working with the blend

The BPC-157 + TB-500 blend is a 10 mg total vial — 5 mg BPC-157 + 5 mg TB-500. Reconstitute with 2 mL for a combined 5 mg/mL (2.5 mg/mL per peptide). The unit math above applies per compound.

5 mg vial — the reference numbers

  • CAS 885340-08-9 · C212H350N56O78S1 · MW 4963.4
  • Purity ≥99.3% (HPLC-MS), lyophilized, -20°C storage

Use the reconstitution calculator for any target dose. See also TB-500 mechanism of action and BPC-157 vs TB-500.

Last updated September 9, 2026

FAQ

Frequently asked questions

How do I reconstitute a 5 mg TB-500 vial?

Add 2 mL of bacteriostatic water for a 2.5 mg/mL stock, or 1 mL for 5 mg/mL. Roll gently — TB-500 dissolves in water and PBS.

What are common research dose ranges for TB-500?

Preclinical protocols commonly use 500 mcg to 2.5 mg per administration in animal models, depending on the study.

How many units is 1 mg from a 2.5 mg/mL stock?

2.5 mg/mL = 2.5 mcg per µL, so 1 mg = 400 µL = 80 units on a U-100 insulin syringe.

Is the TB-500 vial math the same as BPC-157?

Yes — both are 5 mg lyophilized vials, so a 2 mL reconstitution gives 2.5 mg/mL for either.