GHK-Cu Reconstitution and Vial Math: 50 mg Copper Peptide in the Lab

GHK-Cu (copper tripeptide-1) is the copper-bound form of the GHK peptide — the version studied in tissue-regeneration and collagen-synthesis research. It ships as a lyophilized 50 mg vial (10 vials per box), and the reconstitution math follows the same rules as any peptide vial.

Reconstitution table

Water added	Stock concentration	100 mcg	250 mcg	500 mcg
1 mL	50 mg/mL	2 µL	5 µL	10 µL
2 mL	25 mg/mL	4 µL	10 µL	20 µL
5 mL	10 mg/mL	10 µL	25 µL	50 µL

Use bacteriostatic water (0.9% benzyl alcohol). GHK-Cu dissolves readily in water — roll the vial gently, no shaking needed.

Cell-culture notes

GHK-Cu is typically studied at low micromolar working concentrations. The molar weight to remember: 403.9 g/mol for the copper complex — so 403.9 µg/mL ≈ 1 mM. From a 25 mg/mL stock, a 1 mM working solution is a ~1:62 dilution. Use the molecular weight calculator for exact conversions.

The copper makes the difference

Form	Formula	MW
GHK (free peptide)	C14H22N6O4	340.4 g/mol
GHK-Cu (copper complex)	C14H24N6O4Cu	403.9 g/mol

The copper coordination is the biologically relevant form for collagen and glycosaminoglycan synthesis studies — which is why the GLOW blend pairs GHK-Cu with BPC-157 and TB-500 in a single 70 mg research vial.

Reference data

CAS 49557-75-7 · C14H24N6O4Cu · MW 403.9 · 50 mg lyophilized vial · 99.7% purity · -20°C storage

See also GHK-Cu research profile, GHK-Cu vs BPC-157, and the reconstitution calculator.