Semaglutide Reconstitution Math: From 5 mg Vial to Working Stock

Semaglutide ships to research benches as a 5 mg lyophilized cake — and 5 mg is the number that trips people up, because assay work is planned in micrograms per microlitre. This guide walks the arithmetic from vial to working stock.

Concentration first

5 mg = 5,000 µg. Add your chosen diluent volume and the concentration falls out:

• 2 mL diluent → 2,500 µg/mL
• 1 mL diluent → 5,000 µg/mL (doubles every per-volume value)

Smaller volumes mean denser stocks and tinier aliquots; larger volumes are easier to pipette accurately. The semaglutide calculator handles any volume you plan to use.

What each aliquot contains

At a 2,500 µg/mL stock:

• 10 µL holds 25 µg of compound
• a 50 µL aliquot holds 125 µg
• a 100 µL aliquot holds 250 µg

Keep a dilution log — the aliquot size and diluent volume are what make a stored tube traceable months later, long after the vial label is in a drawer.

Vial yield

The planning number for reorder timing is aliquots per vial: 2 mL of recovered stock yields 40 × 50 µL tubes, or 20 × 100 µL. The calculator’s tile shows it live as you change diluent or aliquot size.

Practical notes

Semaglutide is stored lyophilized at −20 °C, reconstituted with USP bacteriostatic water, and used within the window stated on the Certificate of Analysis for the batch. See Semaglutide for the full specification sheet.

For laboratory research use only. Not medical advice. vyrOval supplies this compound as a research chemical and does not recommend, support, or evaluate any administration to humans.