Retatrutide Dosage and Reconstitution Math: 10 mg Vial in the Lab

Retatrutide ships as a lyophilized research peptide in a 10 mg vial (10 vials per box). The vial math is straightforward once you decide on a stock concentration — and it is identical to the math for any 10 mg peptide.

Reconstitution table

Water added	Stock concentration	250 mcg	500 mcg	1 mg
1 mL	10 mg/mL	25 µL (5 units)	50 µL (10 units)	100 µL (20 units)
2 mL	5 mg/mL	50 µL (10 units)	100 µL (20 units)	200 µL (40 units)
3 mL	3.33 mg/mL	75 µL (15 units)	150 µL (30 units)	300 µL (60 units)

Use bacteriostatic water (0.9% benzyl alcohol) for multi-draw research use. Roll the vial to dissolve; retatrutide is soluble in water and dilute acetic acid. Do not shake — lyophilized peptides foam and can shear.

Dose ranges in the research literature

The published phase 2 program (2023) evaluated once-weekly retatrutide across 1, 4, 8 and 12 mg arms over 48 weeks. Weight reduction was dose-dependent, with the 8 mg and 12 mg arms reporting the largest mean reductions. The triple-agonist profile (GIP/GLP-1/glucagon) is the proposed mechanism: incretin-driven appetite suppression plus glucagon-driven energy expenditure.

For in vitro and preclinical work, retatrutide is typically used at concentrations defined by the assay rather than a fixed schedule — reconstitute to a convenient stock and dilute per experiment.

10 mg vial — the reference numbers

• CAS 2381089-83-2 · C221H342N46O68 · MW 4757.7
• Purity ≥99.5% (HPLC-MS), lyophilized, -20°C storage
• Pack: 10 mg vial, 10 vials/box

Use the reconstitution calculator for any water volume and target dose, and the molecular weight calculator when converting molar concentrations. Compare with tirzepatide 10 mg (dual agonist) and semaglutide 5 mg (GLP-1 selective) — different molecules, same vial math.