NAD+: The Redox Coenzyme and Its 500 mg Vial

NAD+ sits at the metabolic center of the catalog — literally. Nicotinamide adenine dinucleotide is the redox coenzyme every energy-metabolism study eventually touches, and its vial is a different scale from the peptides around it.

What the molecule is

NAD+ (C₂₁H₂₇N₇O₁₄P₂, ≈ 663 g/mol) is the oxidized form of the coenzyme that shuttles electrons in hundreds of cellular reactions. Research references concentrate on mitochondrial function, sirtuin activation, and cellular energy metabolism — the pathways that make NAD+ a fixture in aging and metabolic research protocols.

The 500 mg vial is a different game

Most lyophilized products here are 5–10 mg. NAD+ ships at 500 mg, which trips up researchers used to microgram math:

• 500 mg = 500,000 µg
• Reconstitute with 10 mL bacteriostatic water → 50,000 µg/mL
• A 250 mg dose = 5 mL — this is a syringe-and-filter job, not an insulin draw

That’s why the ancillaries section exists: PTFE syringe filters and sterile empty vials are the standard companions when splitting a 500 mg reconstitution into working aliquots.

Aliquoting math

A common workflow is reconstituting then splitting into per-day vials:

1. Reconstitute 500 mg in 10 mL → 50 mg/mL stock
2. Want 5 mL aliquots at 25 mg/mL? Use the dilution calculator: 2.5 mL stock + 2.5 mL bacteriostatic water per vial
3. Store aliquots refrigerated per the Certificate of Analysis

The reconstitution calculator handles the initial concentration step with the NAD+ preset; the dilution calculator takes over when you split.

Practical notes

NAD+ is water-soluble and lyophilized for −20 °C storage. We get asked whether it can be reconstituted at higher volumes for infusion work — the product page lists solubility as water, and the stability window follows the COA. See NAD+ for full specifications.

For laboratory research use only. Not medical advice.